Hexa Biotech

Principle:-
Agar is a Phycocolloid extracted from the red-purple marine algal group (class Rhodophyceae) including Geledium, Pterocladia and Glcilaria. The agar is used as the gelling agent as it remained the firm at the incubation temperatures of bacteria and could not be utilized by microbes. Agar Agar Powder obtained from these algae has desirable features such as melting point of 80??? and Gelling at 42 ??? is best to use as the solidifying agent.

Use:- Recommended to use as a gelling agent in solid medium.

Quality Control

Agar Agar Powder Type I - (Bacteriological) P/N :- RDM-AA-02 Make :- ReadyMed Country of Origin :- India Pack of 100 / 500 Grams

Principle:- Asparagine proline broth is an enrichment broth for Pseudomonas aeruginosa. The composition is strictly mineral base with enantiomeric forms of asparagine and proline, magnesium sulphate, dipotassium sulphate and potassium sulphate. DL-asparagine and proline serve as the sole source of nitrogen. The dipotassium and potassium salts act as a buffer system and magnesium sulfate perform multiple functions, magnesium ion required in a large variety of enzymatic reactions, reproduction and also acts as a buffer. Pseudomonas aeruginosa hydrolyze asparagine to aspartic acid and reproduce vigorously, the appearance of growth with or without fluorescent pigmentation is considered a presumptive test for P. aeruginosa. The microbial count can be determined by using the MPN test and conformation need to be done by subculturing on cetrimide agar. Precautions to be taken:-  Asparagine Proline Broth these microbial media are intended for the in-vitro use only. All the handling, experiments, storage, and discarding should be performed with the help of skilled and knowledgeable technicians and as per the established guidelines. The material should be disposed only after proper sterilization by autoclaving. Please go through the MSDS of the media to avoid any accidents or in emergency. Directions:- Dissolve 14.50 grams in 900 ml distilled water, boil to dissolve the medium completely allow to cool at room temperature and add 25ml of ethanol and dilute up to 1000 ml with distilled water. Distribute as desired in screw-capped bottles. Do not used polypropylene caps with seat. Close the caps so that the seal in the lid just touches the lip of the bottle. Sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 min, cool it to 42-45 °C and inoculate test sample aseptically. Alternatively, sterilize the ethanol by filtration through the cellulose acetate or nitrate membrane of 0.22 µm and then aseptically to the medium after autoclaving and cooling.
Use:- Recommended for the cultivation of Pseudomonas aeruginosa using membrane filter technique.

Additional Information:

• Item Code: RDM-APB-01
• Production Capacity: 100
• Delivery Time: 1 Week
• Packaging Details: Packed in Box

Potato Dextrose Broth is recommended for the isolation and enumeration of yeasts and moulds.Principle:- Potato infusion, serve as the source of all essential nutrients such as amino acids, vitamins, other trace factors which are essential for the growth of microbes and dextrose in it supply the C source and energy source fermenting microbes.Moreover the potato infusion encourages the sporulation and pigment production in some dermatophytes. The potato dextrose Broth can be supplemented with the acid to reduce pH, or the antibiotics for inhibition of bacterial growth.Use:- Recommended for the cultivation of yeasts, and moulds from clinical and non-clinical samples. Also, recommended for the plate count methods for the food, dairy products.Directions:- Suspend 24.0 gram in 1000 ml distilled water adjust the pH if required. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 min. After autoclaving the pH of the medium can be set to 3.5 ±0.2 by addition of the 10% Tartaric acid (Quantity is specified on each container).Performance and Evaluation:- The expected performance of the medium is liable to use as per the direction on the label when stored at optimum conditions and within expiry dateStorage and Shelf Life:- Hygroscopic; Keep container tightly closed. Store at temperatures below 30???C and dry place. Expected performance when stored at optimum conditions and within expiry date.Disposal:- To avoid the contamination or propagation of any hazardous microbes used, unusable or modified preparation of this product must be disposed after autoclaving or incineration after completion of task.

Additional Information:

• Item Code: RDM-PDB-01
• Production Capacity: 100
• Delivery Time: 5-7 Days
• Packaging Details: Box Packaging

Principle:- Sulphate reducing medium (Starkey's medium) is formulated as per APHA (2005). Media is composed of tryptone, soya peptone, sodium chloride, magnesium sulphate, ferrous ammonium sulphate, and sodium lactate. Tryptone and soya peptone provides nitrogen, amino acids and long chain peptides for the growth of microorganisms. Sodium chloride maintains the osmotic balance. Magnesium sulphate and Ferrous ammonium sulphate provides essential ions for growth of sulphate reducing bacteria. Ferrous sulphate is source of sulphate. The sodium lactate is energy source, fermented under anaerobic conditions to produce hydrogen and carbon dioxide. Directions:- Dissolve Sulphate Reducing Medium 29.00 grams in 996 ml distilled water add 4 ml of 60% of sodium lactate solution. Boil to dissolve the medium completely and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C. To prevent oxygen saturation and moisture condensation covers media, with sterile absorbent tops for 10-15 minutes. Maintain the anaerobic conditions properly and inoculate test sample aseptically.
Use:- Recommended for the cultivation of sulphate reducing bacteria.

Additional Information:

• Item Code: RDM-SRM-01
• Production Capacity: 100
• Delivery Time: 1 week
• Packaging Details: Packed in Box

Principle:- MS medium formulated by Murashige and Skoog in 1962 for the tobacco callus culture. It is the blend of inorganic salts (Macro and Micro nutrients), vitamins, amino acids carbohydrates and gelling agent.Macro nutrients serve the source of the Nitrogen, Calcium, Magnesium, Potassium which are essential for the plant growth and metabolism. The micro nutrients Boron, Cobalt, copper, Ferrous, Manganese, Molybdenum, Iodine and Zinc play a crucial role in the plant metabolism. The vitamins such as myo inositol, Nicotinic acid, Pyridoxine and Thiamine acts as the co factor in the universal plant metabolic pathways such as glycolysis and TCA cycle. The glycine serves the source of basic amino acid. The Sucrose serves helps to initiation, normalization and synchronizes the plant cell growth and energy source.

Additional Information:

• Item Code: RDM-MS-01
• Production Capacity: 100
• Delivery Time: 5-7 business days
• Packaging Details: Box Packaging

Principle:- Czapek Dox Broth consists of sucrose, sodium nitrate, dipotassium phosphate, magnesium sulphate, potassium chloride and ferrous sulphate. Sucrose serves as the source of carbon while sodium nitrate serves as source of nitrogen. Dipotassium phosphate buffers the medium. Magnesium sulphate, potassium chloride, ferrous sulphate serves as sources of essential ions. Specimens types analyzed:- Pharmaceutical samples, clinical and non-clinical samples etc.
Use:- Recommended for the general cultivation of fungi from water samples.
Directions:- Dissolve Czapek Dox Broth with Chloramphenicol 35.00 grams in 1000 ml distilled water. Boil to dissolve the medium completely and sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 min, cool it to 42-45 °C and inoculate test sample aseptically.

Specimens types analyzed:- Pharmaceutical samples, clinical and non-clinical samples etc.

Performance and Evaluation:- The expected performance of the medium is liable to use as per the direction on the label when stored at optimum conditions and within expiry date.

Storage and Shelf Life:- Hygroscopic; keep container tightly closed. Store in cool dry place.

Disposal:- To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving after completion of task.

Additional Information:

• Item Code: RDM-CDB-02
• Production Capacity: 100
• Delivery Time: 5-7 Days
• Packaging Details: Packed in Box

• Capitalizing on our state-of-the-art infrastructural facility and cutting edge technology, we are engaged in manufacturing and supplying Ceramic Combustion Boats without Eye (K-99 Grade). These boats are widely used for high temperature applications like metal processing, glass melting, rapid thermal cycling and gemstone purification for jewelry industry. To manufacture these boats, we use optimum quality ceramics. Clients can obtain these Refractory Recrystallized Alumina Combustion Boats Without Eye (K-99 Grade) from us ion different sizes and shapes as per their requirements.
• Can tolerate high temperature. 
• Resistant to breakage.
• High density.
• MATERIAL GRADE: K-99. (Recrystalised Alumina).
• REFRACTORY COMBUSTION BOATS WITHOUT EYE to withstand temperature upto 1800° C.

Additional Information:

• Item Code: HB-RCB-01
• Production Capacity: 50 Units
• Delivery Time: 2 Weeks
• Packaging Details: Box

• Principle:- Aleksandrow broth is consist of salts like mangnesium sulphate, calcium carbonate and potassium alumino silicate, ferric chloride which support the growth of potassium solubilizing bacteria by providing the essential minerals. Dextrose is carbon and energy source. The potassium alumino silicates serve as inorganic source of potassium salts.
• Use:- Recommended for isolation and detection of potassium solubilizing bacteria from soil samples in agro and bio fertilizer industries.
• Directions:- Dissolve 9.60 grams in 1000 ml distilled water check and adjust the pH if required so that after sterilization it is 7.2±0.2. Aleksandrow Broth boil to dissolve the medium completely and sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 min, cool it to 42-45 °C and inoculate test sample aseptically.
• Specimens types analyzed:- Pure cultures, agriculture isolates and soil samples etc.
• Performance and Evaluation:- The expected performance of the medium is liable to use as per the direction on the label when stored at optimum conditions and within expiry date.
• Storage and Shelf Life:- Hygroscopic; keep container tightly closed. Store in cool dry place.
• Disposal:- To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving after completion of task.

Additional Information:

• Item Code: RDM-AKDB-01
• Production Capacity: 100
• Delivery Time: 1 Week
• Packaging Details: Packed in Box

Principle And Interpretation 

Baird Parker Agar was developed by Baird Parker  from the Tellurite-glycine formulation of Zebovitz et al  for isolation and enumeration of Staphylococci in food and other material since it allows a good differentiation of coagulase positive strains. A high correlation has been found between the coagulase test and the presence of clear zone of lipolysis in this medium, which is due to the lecithinase of Staphylococci that breakdown, the egg yolk. On the other hand, studies show that almost 100% of coagulase positive Staphylococci are capable of reducing tellurite, which produces black colonies, whereas other Staphylococci cannot always do so.

Warning and Precautions

In Vitro diagnostic use. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.

Additional Information:

• Item Code: RDM-BPA-01
• Production Capacity: 100
• Delivery Time: 1 Week
• Packaging Details: Packed in Box

CLED agar (cystine lactose electrolyte deficient agar) is a valuable non-inhibitory  growth medium used in the isolation and differentiation of urinary microbes. It contains cystine and lactose and is electrolyte-deficient; the latter trait prevents the swarming of Proteus species. Cystine promotes the formation of cystine-dependent dwarf colonies. Bromothymol blue is the indicator used in the agar, it changes to yellow in case of acid production during fermentation of lactose or changes to deep blue in case of alkalinization. Lactose-positive bacteria build yellow colonies. Bacteria which decarboxylate L-cystine cause an alkaline reaction and build deep blue colonies.

 

Additional Information:

• Item Code: RDM-CLED-01
• Production Capacity: 100
• Delivery Time: 1 Weeks
• Packaging Details: Packed in Box

Principle:- De-Man Roagosa Sharpe agar is described by DeMan, Rogosa and Sharpe (1960). Media composed of proteose peptone, meat extract (equivalent to beef extract), yeast extract, dextrose, sorbitan monooleate, ammonium citrate, sodium acetate magnesium sulfate, manganese sulfate and potassium phosphate dibasic. Proteose peptone, meat extract and yeast extract provide nitrogen, carbon, vitamins and other necessary elements. Dextrose is carbohydrate source, Sorbitan monooleate, ammonium citrate, sodium acetate, magnesium sulfate and manganese sulfate are selective agents inhibits growth of other bacteria and essential for the growth of lactobacilli. Directions:- Dissolve 55.15 grams in 1000 ml distilled water. Boil De-Man Rogosa Sharpe Broth to dissolve the medium completely and sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 min, cool it to 42-45 °C and inoculate test sample aseptically. Incubate in 5% CO2 atmosphere at 33-37°C for 24-72 hrs.
Use:- Recommended for cultivation of Lactobacillus species.

Additional Information:

• Item Code: RDM-MRSB-01
• Production Capacity: 100
• Delivery Time: 7-10 Days
• Packaging Details: Packed in Box

Principle:- Pikovskayas Broth was modified by Sundara Rao and Sinha (1963) for cultivation of phosphate-solubilizing bacteria from soil. It is composed of yeast extract, dextrose, calcium phosphate, ammonium sulphate, potassium chloride, magnesium sulphtate, manganese sulphate and ferrous sulphate. Yeast extract provides nitrogen, carbon, vitamins and other nutrients necessary to support bacterial growth. Dextrose acts as an energy source. Different salts and yeast extract supports the growth of organisms and enhances phosphate solubilization. Calcium phosphate is source of inorganic phosphate.
Use:- Recommended for cultivation of phosphate solubilizing soil microorganisms.

Additional Information:

• Item Code: RDM-PKVB-0001
• Production Capacity: 100
• Delivery Time: 2 Weeks
• Packaging Details: Box

Principle And Interpretation

Haemolysins are exotoxins produced by bacteria that lyse red blood cells. The haemolytic reaction can be visualized on blood agar plates. On blood agar plates colonies of haemolytic bacteria may be surrounded by clear, colourless zone where the red blood cells have been lysed and the haemoglobin destroyed to a colourless compound. This is beta haemolysis. Other types of bacteria can reduce haemoglobin to methaemoglobin which produces a greenish zone around the colonies and is called alpha haemolysis.

Performance and Evaluation

Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.

Additional Information:

• Item Code: RDM-SBA-01
• Production Capacity: 100
• Delivery Time: 1 Week
• Packaging Details: Packed in Box

Principle:- Tryptone soya broth is composed of tryptone, soy peptone, dextrose, sodium chloride and dipotassium phosphate. Tryptone and soy peptone provide nitrogen and other essential nutrients. Dextrose is a carbon source, serves as energy source for optimum growth. Sodium chloride maintains osmotic balance, while di-potassium phosphate is a buffering agent. It is general purpose medium used for cultivation of fastidious and non-fastidious organisms.Use:- Recommended for cultivation of wide variety of microorganism.Directions:- Dissolve tryptic soya broth 30.00 grams in 1000 ml distilled water; boil to dissolve the medium completely and distribute aseptically. Sterilize by autoclaving at 15 lbs. pressure (121 °C) for 15 min, cool it to 42-45 °C and inoculate test sample aseptically.Specimens types analyzed:- Pharmaceutical samples, clinical and non-clinical samples etc.Performance and Evaluation:- The expected performance of the medium is liable to use as per the direction on the label when stored at optimum conditions and within expiry date.Storage and Shelf Life:- Hygroscopic; keep container tightly closed. Store in cool dry place.Disposal:- To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving after completion of task.

Additional Information:

• Item Code: RDM-TrSB-01
• Production Capacity: 100
• Delivery Time: 5-7 Days
• Packaging Details: Box Packaging

• Directions: Remove the cap and inject the test sample aseptically.
• Disposal: To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving or incineration after completion of task.
• Do not use contaminated or deteriorated bottles.
• For laboratory use only.
• Product description: Expected performance when stored at optimum conditions and within the expiry date. No liability accepted for accidents in handling or use.

Additional Information:

• Item Code: RDM-IB-TRSB-01
• Production Capacity: 100
• Delivery Time: 5-7 Business Days
• Packaging Details: Box Packaging

• Principle:- Tryptone bile agar is another differential medium which specifically allows the Indole positive, late lactose fermenting E. coli. The Tyrptophan of Tryptone mediates the production of Indole which further can be detected by the application of the Kovacs or Ehrlichs reagent. While the other organisms get inhibited by the presence of Bile salts and incubating at elevated temperature of 44 C. The TBA is used for detection of E. coli by direct plating method over the Most Probable Number (MPN) Method.
• Use:- Recommended for rapid detection and enumeration of Escherichia coli by modified direct platting method.
• Directions:- Suspend 36.5 gram in 1000 ml distilled water adjust the pH if required. Boil to dissolve the medium completely. Tryptone Bile Agar sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 min, cool it to 42-45 °C and distribute aseptically in to the plates. Ensure complete solidification and inoculate aseptically.
• Specimens types analyzed:- Water samples, raw meat, milk and dairy, food samples, etc.
• Performance and Evaluation:- The expected performance of the medium is liable to use as per the direction on the label when stored at optimum conditions and within expiry date.
• Storage and Shelf Life:- Hygroscopic; Keep container tightly closed. Store at temperature below 30 C and dry place. Expected Performance when stored at optimum and within expiry date.
• Disposal:- To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving or incineration after completion of task.

Additional Information:

• Item Code: RDM-TBA-01
• Production Capacity: 100
• Delivery Time: 5-7 Days
• Packaging Details: Packed in Box

• Violet Red Bile Agar is used for the enumeration of coliforms in food and dairy products in a laboratory setting. VRBA is a selective medium for the detection and enumeration of coliform organisms. 
• The medium has been used for the determination of the coli-aerogenes content of water, milk and other dairy products, dairy equipment, and food products etc.

Additional Information:

• Item Code: RDM-VRBA-01
• Production Capacity: 100
• Delivery Time: 5-7 Days
• Packaging Details: Packed in Box

• Use:- Recommended for culturing yeasts and molds from food and dairy products.
• Directions:- Dissolve Ashby’s Mannitol Broth 50.00 grams in 1000 ml distilled water. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C and inoculate test sample aseptically.
• Specimens’ types analyzed:- Pharmaceutical samples, clinical and non-clinical samples, food and dairy products etc.
• Performance and Evaluation:- The expected performance of the medium is liable to use as per the direction on the label when stored at optimum conditions and within expiry date.
• Disposal:- To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving after completion of task.
• Principle:- Fungal broth is composed of soya peptone and dextrose used for cultivation of fungi. Soya peptone, serve as the source of nitrogen, amino acids, vitamins, other trace elements, required for the growth of fungi and molds. Dextrose supply carbon and energy source. The acidic pH makes the medium partially selective for the growth of fungi and inhibits the growth of bacteria. Storage and Shelf Life:- Hygroscopic; keep container tightly closed. Store in cool dry place.

Additional Information:

• Item Code: RDM-ASMB-01
• Production Capacity: 100
• Delivery Time: 5-7 Days
• Packaging Details: Packed in box

Principle And Interpretation:-
Brilliant Green Agar medium is recommended as a primary plating medium for isolation of Salmonella species was first described by Kristensen et al as medium for differentiation of paratyphoid B from other Gram negative enteric bacteria. Kauffmann further modified it for isolation of Salmonella from stool samples. Brilliant green agar is also recommended by APHA FDA and is in accordance with United States Pharmacopoeia. This medium is employed in testing clinical specimens. Heavy inocula and heavily contaminated samples can be analyzed due to the outstanding selectivity of this medium. Brilliant Green Agar is used in the microbial limits test and with novobiocin for testing food and pharmaceutical products.

Additional Information:

• Item Code: RDM-BGA-01
• Production Capacity: 100
• Delivery Time: 5-7 Days
• Packaging Details: Packed in Box

• Directions:- Dissolve 36.00 grams in 1000 ml distilled water Boil to dissolve the medium completely and sterilize by autoclaving at 15 lbs pressure (121 'C) for 15 min, cool it to 42-45 'C and distribute aseptically. Ensure complete solidification and inoculate test sample aseptically. Disposal:- To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving or incineration after completion of task.
• Use :- Recommended for the differential isolation of the gram-negative Enteric Bacilli from clinical and non-clinical samples.
• Expected Performance when stored at optimum conditions and within the expiry date.
• No liability accepted for accidents in handling or use.
• Hygroscopic; Keep container tightly closed. Store in cool (10-30'C) & Dry place.
• For Laboratory, Institutional & Industrial use only. Not for Human Consumption.

Additional Information:

• Item Code: RDM-EMBA-01
• Production Capacity: 100
• Delivery Time: 5-7 Days
• Packaging Details: Box Packaging

Directions:- Suspend 13.0 gram in 1000 ml distilled water adjust the pH if required. Lactose Broth Base sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 min, cool it to 42-45 °C.

Specimens types analyzed:- Water samples, Sewage, Food and dairy samples,
etc.

Performance and Evaluation:- The expected performance of the medium is liable to use as per the direction on the label when stored at optimum conditions and within expiry date.

Disposal:- To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving or incineration after completion of task.

Storage and Shelf Life:- Hygroscopic; Keep container tightly closed. Store at temperature below 30???C and Dry place Expected Performance when stored at optimum and within expiry date.

Principle:- Peptone and Meat extract serves as the source of all essential nutrients such as amino acids, vitamins, other trace factors which are essential for the growth of microbes. The lactose in it plays a crucial role for selection of lactose fermenting microbes.

 

Use:- Recommended for selective isolation and differentiation of Lactose fermenting and Lactose non-fermenting bacteria from water, sewage etc. Specifically the coliforms; E coli by using Durham’s tube for detection of gas production. The Lactose Broth is recommended for the use in microbial examination of water samples, milk and dairy products, and recommended by many regulatory bodies such as BIS (Burro Of Indian Standards).

Additional Information:

• Item Code: RDM-LB-01
• Production Capacity: 100
• Delivery Time: 10-12 Business Days
• Packaging Details: Box Packaging

Principle:- The presence of Bile salts, Sodium citrate and Brilliant green inhibit the gram positive bacteria most of coliforms and limiting Proteus spp. and allowing the Salmonella spp. to grow. The sodium thiosulphate and the ferric citrate detect the H2S production giving black centered colonies of Salmonella spp. While the Shigella spp. does not produce H2S gas resulting in the formation of colorless colonies. The some of the Escherichia strains grow on the SS agar, giving pink to colorless. Proteose peptone provides all the essential amino acids and vitamins and other nutrients.

Use:- Recommended for the selective isolation and determination of Salmonella and some Shigella sp. from clinical and non-clinical samples.

Directions:- Suspend 60.0 gram in 1000 ml distilled water adjust the pH if required. Boil to dissolve salmonella shigella agar the medium completely. DO NOT AUTOCLAVE, cool it to 42-45 °C and distribute aseptically in to the plates. Ensure complete solidification and inoculate aseptically.

Storage and Shelf Life:- Hygroscopic; Keep container tightly closed. Store at temperatures below 30???C and dry place. Expected performance when stored at optimum conditions and within expiry date.

Salmonella Shigella Agar Disposal:- To avoid the contamination or propagation of any hazardous microbes used, unusable or modified preparation of this product must be disposed after autoclaving or incineration after completion of task.

 

Performance and Evaluation:- The expected performance of the medium is liable to use as per the direction on the label when stored at optimum conditions and within expiry date.

Additional Information:

• Item Code: RDM-SSA-01
• Production Capacity: 100
• Delivery Time: 5-7 business days
• Packaging Details: Box Packaging

Principle:- Tryptone and Soya peptone provides nitrogen, vitamins and other essential nutrition, sodium chloride maintains the osmotic balance, while dextrose serves the energy source and Di-potassium phosphate buffers the media. It supports the growth of both fastidious as well as non-fastidious organisms. It also supports the growth of aerobic as well as anaerobic organisms.

Use:- Recommended for the routine enrichment, culturing and maintenance of Salmonella typhi.

Directions:- Suspend 45.0 gram in 1000 ml distilled water adjust the pH if required. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C and distribute aseptically in to the plates. Tryptic Soy Agar ensure complete solidification and inoculate aseptically.

Specimens types analyzed:- Water samples, raw meat, milk and dairy, food samples, etc.

Performance and Evaluation:- The expected performance of the medium is liable to use as per the direction on the label when stored at optimum conditions and within expiry date.

Storage and Shelf Life:- Hygroscopic; keep container tightly closed. Store at temperature below 30???C and dry place. Expected performance when stored at optimum conditions and within expiry date.

Disposal:- To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving or incineration after completion of task.

 

Additional Information:

• Item Code: RDM-TrSA-01
• Production Capacity: 500
• Delivery Time: 1 Week
• Packaging Details: in HDPE Bottles & Can

Principle:- These mediums contain malic acid which serves as the prime carbon source and favors the growth of Azospirillum and prevent the overgrowth of other nitrogen fixers. Dipotassium phosphates help to maintain the pH of medium. Necessary trace elements like ferrous Sulphate, manganese Sulphate, sodium molybdate support growth of Azospirillum species. Divalent salts like calcium and magnesium helps metabolism of cells. Sodium chloride helps to maintain osmotic balance of cell. Due to the nitrogen fixation by diazotroph bacteria ammonia is produced, the color of the medium changed from light yellow color to greenish blue or blue (Bromothymol blue pH indicator).Use:- Recommended for cultivation of Azospirillum species.Azospirillum Medium w/o Agar Directions:-

• Part A:- Dissolve 6.33 grams in 950 ml distilled water, boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 min, cool it to 42-45 °C.
• Part B:- Dissolve 4 grams of part B in 50 ml of sterile distilled water. Mix 950 ml of part A with 50 ml of part B in 1 liter sterile container, mix well and dispense into sterile tubes or flasks as desired.

Specimens types analyzed:- Soil samples etc.Performance and Evaluation:- The expected performance of the medium is liable to use as per the direction on the label when stored at optimum conditions and within expiry date.Storage and Shelf Life:- Hygroscopic; keep container tightly closed. Store in cool dry place.Disposal:- To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving after completion of task.

Additional Information:

• Item Code: RDM-AZMB-01
• Production Capacity: 100
• Delivery Time: 5-7 Days
• Packaging Details: Packed in Box

• Principle:- Agar is a Phycocolloid extracted from the red-purple marine algal group (class Rhodophyceae) including Geledium, Pterocladia and Glcilaria. The agar is used as the gelling agent as it remained the firm at the incubation and long period incubation of Plant tissue culture media. Agar obtained from these algae has desirable features such as melting point of 80 and Gelling at 40  is best to use as the solidifying agent.
• Use:- Recommended to use as a gelling agent in solid medium.
• Storage and Shelf Life:- Hygroscopic; keep container tightly closed. Agar Agar powder (PTC Grade) store at temperatures below 30 C and dry place. Expected performance when stored at optimum conditions and within expiry date.
• Disposal:- To avoid the contamination or propagation of any hazardous microbes used, unusable or modified preparation of this product must be disposed after autoclaving or incineration after completion of task.

Additional Information:

• Item Code: RDM-AA-02
• Production Capacity: 100
• Delivery Time: 1 Week
• Packaging Details: Box Packaging

Principle

• EMB agar is characterized by the presence of a combination of the two dyes eosin and methylene blue in the ratio of 6:1.
• Gram-negative bacteria that ferment the lactose produce acid which lowers the pH. This encourages dye absorption by the colonies and turns the colonies dark purple as the acid acts upon the dyes.
• In addition, certain lactose-fermenting bacteria produce flat, dark colonies with a green metallic sheen.
• Other lactose fermenters produce larger, mucoid colonies, often purple only in their center.
• In EMB agar, most of the strains of  E. coli colonies have a characteristic green sheen. Rapid fermentation of lactose & production of strong acids, thus a rapid reduction in the pH of the EMB agar the critical factor in the formation of the green metallic sheen observed with E. coli, rapid fermentation of lactose and formation of strong acids.

Additional Information:

• Item Code: RDM-EMB-01
• Production Capacity: 100
• Delivery Time: 5-10 Days
• Packaging Details: Box Packaging